Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors
SciencePublished 20 April 2017
Alexandra–Chloé Villani, Rahul Satija, Gary Reynolds, Siranush Sarkizova, Karthik Shekhar, James Fletcher
Citations2,488
SJR quartileQ1
SJR score10.42
SNIP6.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveWill appear once the snapshot is generated
MethodsWill appear once the snapshot is generated
PopulationWill appear once the snapshot is generated
Sample sizeWill appear once the snapshot is generated
OutcomesWill appear once the snapshot is generated
ResultsWill appear once the snapshot is generated
LimitationsWill appear once the snapshot is generated
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
This refined analysis has identified, among others, a previously unknown dendritic cell population that potently activates T cells and reclassify pDCs as the originally described “natural interferon-producing cells (IPCs)” with weaker T cell proliferation induction ability.
Abstract
What's in a drop of blood? Blood contains many types of cells, including many immune system components. Immune cells used to be characterized by marker-based assays, but now classification relies on the genes that cells express. Villani et al. used deep sequencing at the single-cell level and unbiased clustering to define six dendritic cell and four monocyte populations. This refined analysis has identified, among others, a previously unknown dendritic cell population that potently activates T cells. Further cell culture revealed possible differentiation progenitors within the different cell populations. Science , this issue p. eaah4573
Keywords
Immunology and MicrobiologyBiochemistry, Genetics and Molecular Biology
Nucleic Acids ResearchKEGG: Kyoto Encyclopedia of Genes and Genomes
39,218 Citations2000Minoru Kanehisa
Journal of Machine Learning ResearchVisualizing Data using t-SNE
35,654 Citations2008Laurens van der Maaten, Geoffrey E. Hinton
A new technique called t-SNE that visualizes high-dimensional data by giving each datapoint a location in a two or three-dimensional map, a variation of Stochastic Neighbor Embedding that is much easier to optimize, and produces significantly better visualizations by reducing the tendency to crowd points together in the center of the map.
Nucleic Acids ResearchKEGG: Kyoto Encyclopedia of Genes and Genomes
32,426 Citations1999Hiroyuki Ogata, Susumu Goto +4 more
KEGG maintains the GENES database for the gene catalogs of all organisms with complete genomes and selected organisms with partial genomes, as well as the LIGAND database for chemical compounds and enzymes.
ScienceTissue-based map of the human proteome
15,936 Citations2015Mathias Uhlén, Linn Fagerberg +34 more
A genome-wide analysis of the tissue specificity of RNA and protein expression covering more than 90% of the putative protein-coding genes is reported, complemented with analyses of various subproteomes, such as predicted secreted proteins and membrane-bound proteins.
Nucleic Acids ResearchKEGG as a reference resource for gene and protein annotation
7,858 Citations2015Minoru Kanehisa, Yoko Sato +3 more
The KEGG GENES database now includes viruses, plasmids, and the addendum category for functionally characterized proteins that are not represented in complete genomes, and new automatic annotation servers, BlastKOalA and GhostKOALA, are made available utilizing the non-redundant pangenome data set generated from theGENES database.
Nature BiotechnologyThe dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells
7,845 Citations2014Cole Trapnell, Davide Cacchiarelli +8 more
Monocle is described, an unsupervised algorithm that increases the temporal resolution of transcriptome dynamics using single-cell RNA-Seq data collected at multiple time points that revealed switch-like changes in expression of key regulatory factors, sequential waves of gene regulation, and expression of regulators that were not known to act in differentiation.
CellHighly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
7,828 Citations2015Evan Z. Macosko, Anindita Basu +14 more
Drop-seq will accelerate biological discovery by enabling routine transcriptional profiling at single-cell resolution by separating them into nanoliter-sized aqueous droplets, associating a different barcode with each cell's RNAs, and sequencing them all together.
Nature CommunicationsMassively parallel digital transcriptional profiling of single cells
7,821 Citations2017Grace Zheng, Jessica M. Terry +31 more
A new droplet-based single cell RNA-seq platform capable of processing tens of thousands of cells across 8 independent samples in minutes is described, and cellular subtypes and host–donor chimerism in transplant patients are demonstrated.
BMC BioinformaticsGOrilla: a tool for discovery and visualization of enriched GO terms in ranked gene lists
3,674 Citations2009Eran Eden, Roy Navon +3 more
Nature MethodsSmart-seq2 for sensitive full-length transcriptome profiling in single cells
2,580 Citations2013Simone Picelli, Åsa K. Björklund +4 more
Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells to improve detection, coverage, bias and accuracy.
CellData-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis
2,564 Citations2015Jacob Levine, Erin F. Simonds +14 more
Using hematopoietic progenitors, a signaling-based measure of cellular phenotype was defined, which led to isolation of a gene expression signature that was predictive of survival in independent cohorts, yielding insights into AML pathophysiology.
Science<i>Batf3</i> Deficiency Reveals a Critical Role for CD8α <sup>+</sup> Dendritic Cells in Cytotoxic T Cell Immunity
2,032 Citations2008Kai Hildner, Brian T. Edelson +11 more
An important role is suggested for CD8α+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection in Batf3–/– mice.
Nature reviews. ImmunologyDendritic cells, monocytes and macrophages: a unified nomenclature based on ontogeny
1,721 Citations2014Martin Guilliams, Florent Ginhoux +7 more
This Opinion article suggests that the mononuclear phagocyte system can be classified primarily by their ontogeny and secondarily by their location, function and phenotype, which permits a more robust classification during both steady-state and inflammatory conditions.
Nature MedicinePlasmacytoid monocytes migrate to inflamed lymph nodes and produce large amounts of type I interferon
1,715 Citations1999Marina Cella, David Jarrossay +5 more
Results, with the distinct cell phenotype, indicate that plasmacytoid monocytes represent a specialized cell lineage that enters inflamed lymph nodes at high endothelial venules, where it produces type I interferon.
Nature Reviews GeneticsA census of human transcription factors: function, expression and evolution
1,607 Citations2009Juan M. Vaquerizas, Sarah Kummerfeld +2 more
An analysis of 1,391 manually curated sequence-specific DNA-binding transcription factors, their functions, genomic organization and evolutionary conservation provides a solid foundation for future investigations to elucidate regulatory mechanisms underlying diverse mammalian biological processes.
Nature BiotechnologyComputational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells
1,300 Citations2015Florian Buettner, Kedar Nath Natarajan +7 more
It is shown that the single-cell latent variable model (scLVM) allows the identification of otherwise undetectable subpopulations of cells that correspond to different stages during the differentiation of naive T cells into T helper 2 cells.
The Journal of Experimental MedicineThe Enigmatic Plasmacytoid T Cells Develop into Dendritic Cells with Interleukin (IL)-3 and CD40-Ligand
1,178 Citations1997Géraldine Grouard, Marie‐Clotilde Rissoan +4 more
It is demonstrated that CD4+ CD11c−CD3− cells, isolated from tonsils, correspond to the so-called plasmacytoid T cells, an obscure cell type that has long been observed by pathologists within secondary lymphoid tissues.
The European Physical Journal BA smart local moving algorithm for large-scale modularity-based community detection
1,158 Citations2013Ludo Waltman, Nees Jan van Eck
It is shown that the proposed smart local moving algorithm identifies community structures with higher modularity values than other algorithms for large-scale modularity optimization, among which the popular “Louvain algorithm”.
Nature reviews. ImmunologySiglec-mediated regulation of immune cell function in disease
1,127 Citations2014Matthew S. Macauley, Paul R. Crocker +1 more
The recent advances in understanding of the roles of Siglecs in the regulation of immune cell function in infectious diseases, inflammation, neurodegeneration, autoimmune diseases and cancer are described.
Nature reviews. ImmunologyThe multifaceted biology of plasmacytoid dendritic cells
1,080 Citations2015Melissa Swiecki, Marco Colonna
Recent progress in the field of pDC biology is summarized, focusing on the molecular mechanisms that regulate the development and functions of p DCs, the pathways involved in their sensing of pathogens and endogenous nucleic acids, their functions at mucosal sites, and their roles in infection, autoimmunity and cancer.
NatureSingle-cell RNA-seq reveals dynamic paracrine control of cellular variation
1,063 Citations2014Alex K. Shalek, Rahul Satija +20 more
This study highlights the importance of cell-to-cell communication in controlling cellular heterogeneity and reveals general strategies that multicellular populations can use to establish complex dynamic responses.
The Journal of Experimental MedicineHuman CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens
989 Citations2010Sarah L. Jongbloed, Andrew J. Kassianos +11 more
The data demonstrate a role for CD141+ DCs in the induction of cytotoxic T lymphocyte responses and suggest that they may be the most relevant targets for vaccination against cancers, viruses, and other pathogens.
Genome ResearchDefining cell types and states with single-cell genomics
889 Citations2015Cole Trapnell
These measurements may finally make explicit the metaphor that C.H. Waddington posed nearly 60 years ago to explain cellular plasticity, and enable not only precise characterization of cell state, but also a stunningly high-resolution view of transitions between states.
Cell stem cellHematopoiesis: A Human Perspective
862 Citations2012Sergei Doulatov, Faiyaz Notta +2 more
This work highlights 2 decades of studies focusing on isolation and molecular regulation of human HSCs, therapeutic applications, and early lineage commitment steps, and compares mouse and humanized models to identify both conserved and species-specific mechanisms that will aid future preclinical research.
ImmunityIRF4 Transcription Factor-Dependent CD11b+ Dendritic Cells in Human and Mouse Control Mucosal IL-17 Cytokine Responses
807 Citations2013Andreas Schlitzer, Naomi McGovern +27 more
The demonstration of mouse and human DC subsets specialized in driving IL-17 responses highlights the conservation of key immune functions across species and will facilitate the translation of mouse in vivo findings to advance DC-based clinical therapies.
Nature ImmunologyDeciphering the transcriptional network of the dendritic cell lineage
776 Citations2012Jennifer C. Miller, Brian D. Brown +18 more
A transcriptional program expressed specifically during the steady-state migration of tissue DCs to the draining lymph nodes that may control tolerance to self tissue antigens is identified and predicted regulators of DC functional diversity in tissues are predicted.
ImmunityDevelopment and Function of Dendritic Cell Subsets
755 Citations2014Alexander Mildner, Steffen Jung
Recent progress in the understanding of cDC subset ontogeny and transcription factor dependencies are reviewed, as well as emerging functional specializations within the cDC compartment in lymphoid and nonlymphoid tissues are discussed.
ScienceDistinct routes of lineage development reshape the human blood hierarchy across ontogeny
753 Citations2015Faiyaz Notta, Sasan Zandi +12 more
Using a cell-sorting scheme based on markers linked to Er and Mk lineage specification, it is found that previously described populations of multipotent progenitors (MPPs), CMPs, and megakaryocyte-erythroid progenitor (MEPs) were heterogeneous and could be further purified.
ImmunityHuman Tissues Contain CD141hi Cross-Presenting Dendritic Cells with Functional Homology to Mouse CD103+ Nonlymphoid Dendritic Cells
694 Citations2012Muzlifah Haniffa, Amanda Shin +26 more
A CD141hi DC present in human interstitial dermis, liver, and lung that was distinct from the majority of CD1c+ and CD14+ tissue DCs and superior at cross-presenting soluble antigens was identified.
The Journal of Experimental MedicineCharacterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8α+ dendritic cells
647 Citations2010Lionel Franz Poulin, Mariolina Salio +15 more
The characterization of human DNGR-1+ BDCA3hi DCs and the ability to grow them in vitro opens the door for exploiting this subset in immunotherapy.
BioinformaticsIdentification of cell types from single-cell transcriptomes using a novel clustering method
617 Citations2015Chen Xu, Zhengchang Su
A novel algorithm named SNN-Cliq is described that clusters single-cell transcriptomes using the concept of shared nearest neighbor that shows advantages in handling high-dimensional data.
The Journal of Experimental MedicineThe XC chemokine receptor 1 is a conserved selective marker of mammalian cells homologous to mouse CD8α+ dendritic cells
591 Citations2010Karine Crozat, Rachel Guiton +12 more
XCR1 constitutes the first conserved specific marker for cell subsets homologous to mouse CD8α+ DCs in higher vertebrates and promotes their ability to activate early CD8+ T cell defenses against an intracellular pathogenic bacteria.
ImmunityIRF4 Transcription-Factor-Dependent CD103+CD11b+ Dendritic Cells Drive Mucosal T Helper 17 Cell Differentiation
574 Citations2013Emma K. Persson, Heli Uronen‐Hansson +9 more
It is demonstrated that intestinal CD103(+)CD11b(+) DC survival was dependent on interferon regulatory factor 4 (IRF4), and results suggest a central role for IRF4-dependent, IL-6 producing CD103 (+) CD11b (+) DCs in intestinal Th17 cell differentiation.
CellDesign and Analysis of Single-Cell Sequencing Experiments
568 Citations2015Dominic Grün, Alexander van Oudenaarden
This Primer provides an overview of available methods and discusses experimental design and single-cell data analysis and hopes that these guidelines will enable a growing number of researchers to leverage the power of single- cell sequencing.
Cell stem cellExpression Analysis Highlights AXL as a Candidate Zika Virus Entry Receptor in Neural Stem Cells
529 Citations2016Tomasz J. Nowakowski, Alex A. Pollen +4 more
Examining the expression of receptors implicated in cell entry of several enveloped viruses including ZIKV across diverse cell types in the developing brain found that the candidate viral entry receptor AXL is highly expressed by human radial glial cells, astrocytes, endothelial cells, and microglia in developing human cortex and by progenitor cells in developing retina.
ImmunityNotch2 Receptor Signaling Controls Functional Differentiation of Dendritic Cells in the Spleen and Intestine
518 Citations2011Kanako L. Lewis, Michele L. Caton +12 more
Notch2 deletion led to the loss of CD11b(+)CD103(+) DCs in the intestinal lamina propria and to a corresponding decrease of IL-17-producing CD4(+) T cells in the intestine, indicating that Notch2 is a common differentiation signal for T cell-priming CD 11b(+) DC subsets in the spleen and intestine.
Annual Review of ImmunologyTAM Receptor Signaling in Immune Homeostasis
514 Citations2015Carla V. Rothlin, Eugenio Antonio Carrera Silva +2 more
The important milestones in the discovery of these RTKs and their ligands and the studies that underscore the functional importance of this signaling pathway in physiological immune settings and disease are reviewed.
PLoS ONEA Mass Spectrometric-Derived Cell Surface Protein Atlas
448 Citations2015Damaris Bausch‐Fluck, Andreas Hofmann +18 more
Nature ImmunologyNotch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens
443 Citations2013Ansuman T. Satpathy, Carlos G. Briseño +21 more
A nonredundant function for CD11b+ cDCs in the response to pathogens in vivo is demonstrated, demonstrating immunity to the attaching-and-effacing pathogen Citrobacter rodentium and demonstrating the role of the signaling receptor Notch2 in cDC differentiation.
BioinformaticsData exploration, quality control and testing in single-cell qPCR-based gene expression experiments
442 Citations2012Andrew McDavid, Greg Finak +6 more
A statistical model accounting for the fact that genes at the single-cell level can be on (and a continuous expression measure is recorded) or dichotomously off (and the recorded expression is zero) is proposed and a combined likelihood ratio test for differential expression that incorporates both the discrete and continuous components is derived.
Nature ImmunologyRe(de)fining the dendritic cell lineage
423 Citations2012Ansuman T. Satpathy, Xiaodi Wu +2 more
Progress has been made in three critical areas of DC biology: the characterization of lineage-restricted progenitors in the bone marrow, the identification of cytokines and transcription factors required during differentiation, and the development of genetic tools for the visualization and depletion of DCs in vivo.
Nature MedicineA novel dendritic cell subset involved in tumor immunosurveillance
397 Citations2006Julien Taı̈eb, Nathalie Chaput +23 more
Adoptive transfer of these IFN-producing killer dendritic cells into tumor-bearing Rag2/−Il2rg−/− mice prevented tumor outgrowth, whereas transfer of conventional NK cells did not and was identified as pivotal sensors and effectors of the innate antitumor immune response.
Nature MedicineInterferon-producing killer dendritic cells provide a link between innate and adaptive immunity
392 Citations2006Camie W. Chan, Emily B. Crafton +10 more
In vivo, splenic IKDCs preferentially show NK function and, upon systemic infection, migrate to lymph nodes, where they primarily show antigen-presenting cell activity, which formally distinguish them from classical NK cells.
Cancer ResearchNatural Human Plasmacytoid Dendritic Cells Induce Antigen-Specific T-Cell Responses in Melanoma Patients
342 Citations2013Jurjen Tel, Erik H.J.G. Aarntzen +13 more
The results indicate that vaccination with naturally occurring pDC is feasible with minimal toxicity and that in patients with metastatic melanoma, it induces favorable immune responses.
Current Protocols in Molecular BiologyPreparation of Single‐Cell RNA‐Seq Libraries for Next Generation Sequencing
316 Citations2014John J. Trombetta, David Gennert +4 more
This unit describes a method that merges several important technologies to produce, in high‐throughput, single‐cell RNA‐Seq libraries, the heterogeneity that exists within these populations of transcriptomics.
Cancer CellThe Public Repository of Xenografts Enables Discovery and Randomized Phase II-like Trials in Mice
304 Citations2016Elizabeth C. Townsend, Mark A. Murakami +95 more
It is demonstrated that large studies of acute leukemia PDXs that mimic human randomized clinical trials can characterize drug efficacy and generate transcriptional, functional, and proteomic biomarkers in both treatment-naive and relapsed/refractory disease.
BioinformaticsStatistical significance of variables driving systematic variation in high-dimensional data
272 Citations2014Neo Christopher Chung, John D. Storey
The jackstraw is introduced, a new approach called the Jackstraw that allows one to accurately identify genomic variables that are statistically significantly associated with any subset or linear combination of PCs and can greatly simplify complex significance testing problems encountered in genomics.
The Journal of Experimental MedicineRestricted dendritic cell and monocyte progenitors in human cord blood and bone marrow
247 Citations2015Jaeyop Lee, Gaëlle Breton +8 more
The differentiation of human progenitor cells into dendritic cells (DCs) is tracked and it is shown that a granulocyte/monocyte/DC progenitors gives rise to a monocyte-DC progensitor that in turn gives Rise to both monocytes and a common DC progenator.
The Journal of Experimental MedicineDifferential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation
242 Citations2009Muzlifah Haniffa, Florent Ginhoux +11 more
Observations suggest that dermal macrophage and DC are from distinct lineages and that persistent recipient macrophages, although unlikely to initiate alloreactivity, may contribute to GVHD by sustaining the responses of previously activated T cells.
Cell ReportsSimultaneous Multiplexed Measurement of RNA and Proteins in Single Cells
229 Citations2015Spyros Darmanis, Caroline J. Gallant +10 more
This work presents an approach to simultaneously and specifically interrogate large sets of protein and RNA targets in lysates from individual cells, enabling investigations of cell functions and responses.
The Journal of ImmunologyCD2 Distinguishes Two Subsets of Human Plasmacytoid Dendritic Cells with Distinct Phenotype and Functions
202 Citations2009Toshimichi Matsui, John E. Connolly +10 more
It is shown that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion.
The Journal of Experimental MedicineCirculating precursors of human CD1c+ and CD141+ dendritic cells
192 Citations2015Gaëlle Breton, Jaeyop Lee +9 more
The Liu and Nussenzweig groups identify the immediate precursor of CD1c+ and CD141+ dendritic cells in the circulation of healthy donors.
Current Opinion in ImmunologyDefining dendritic cells
183 Citations2014Barbara U. Schraml, Caetano Reis e Sousa
Other approaches to try and solve questions of DC lineage attribution are reviewed with an emphasis on recent insights arising from increased understanding of DC ontogeny and differentiation.
LeukemiaMolecular profiling of blastic plasmacytoid dendritic cell neoplasm reveals a unique pattern and suggests selective sensitivity to NF-kB pathway inhibition
178 Citations2014Maria Rosaria Sapienza, Fabio Fuligni +24 more
A molecular signature representative of the transcriptional abnormalities of BPDCN is identified and a cellular model proposing a novel therapeutic approach in the setting of this otherwise incurable disease is developed.
Proceedings of the National Academy of SciencesCX <sub>3</sub> CR1 <sup>+</sup> CD8α <sup>+</sup> dendritic cells are a steady-state population related to plasmacytoid dendritic cells
157 Citations2010Liat Bar‐On, Tal Birnberg +8 more
The expression-profiling data of this study refine the resolution of previous DC definitions, sharpen the border of classical CD8 α+ and CD8α− DC, and should assist the identification of human counterparts of murine DC subsets.
Annual Review of ImmunologyA Genomic Approach to Human Autoimmune Diseases
152 Citations2010Virginia Pascual, Damien Chaussabel +1 more
More quantitative and sensitive high-throughput RNA profiling methods are starting to be available and will be necessary for transcriptome analyses to become routine tests in the clinical setting, and it is expected that this to crystallize within the coming decade.
Seminars in Cell and Developmental BiologyHuman mononuclear phagocyte system reunited
119 Citations2015Muzlifah Haniffa, Venetia Bigley +1 more
The current classification of human mononuclear phagocytes and the parallel organization of this network in the mouse is discussed and the genetic control and developmental pathway of human Mononuclear Phagocyte subsets is reviewed and the immunological functions of the distinct subsets in health and inflammation are reviewed.
BloodHuman plasmacytoid dendritic cells are equipped with antigen-presenting and tumoricidal capacities
110 Citations2012Jurjen Tel, Evelien Smits +4 more
It is shown that human pDCs activated by the preventive vaccine FSME specifically up-regulate CD56 on their surface, a marker that was thought to be specific for NK cells and associated with cytolytic effector functions, and revealed that p DCs attained a mature phenotype, secreted proinflammatory cytokines, and had the capacity to present antigens and stimulate T cells.
British Journal of HaematologyPlasmacytoid dendritic cell leukaemia/lymphoma: towards a well defined entity?
100 Citations2007Francine Garnache‐Ottou, Jean Feuillard +1 more
Cancer ControlBlastic Plasmacytoid Dendritic Cell Neoplasm: Update on Molecular Biology, Diagnosis, and Therapy
84 Citations2014Wasif Riaz, Ling Zhang +2 more
Although BPDCN is initially sensitive to conventional chemotherapy regimens, this response is relatively short and long-term prognosis is poor, and novel targeted therapies may improve outcomes for patients with BPD CN.
BloodInterferon-producing killer dendritic cells (IKDCs) arise via a unique differentiation pathway from primitive c-kitHiCD62L+ lymphoid progenitors
82 Citations2007Robert S. Welner, Rosana Pelayo +6 more
Observations suggest that IKDCs may arise from a unique differentiation pathway, and one that diverges early from those responsible for NK cells, pDCs, and T and B cells.
Nature ProtocolsDefining human dendritic cell progenitors by multiparametric flow cytometry
71 Citations2015Gaëlle Breton, Jaeyop Lee +2 more
This protocol provides optimized antibody panels, as well as gating strategies, for immunostaining of BM and cord blood specimens to study human DC hematopoiesis in health, disease and vaccine settings.
The Journal of Experimental MedicineLeukemia-associated activating mutation of Flt3 expands dendritic cells and alters T cell responses
66 Citations2016Colleen M. Lau, Simone A. Nish +4 more
It is shown that the FLT3-ITD mutation directly affects dendritic cell development in preleukemic mice, indirectly modulating T cell homeostasis and supporting the expansion of regulatory T cells.
The Journal of ImmunologyAn Imbalance of Two Functionally and Phenotypically Different Subsets of Plasmacytoid Dendritic Cells Characterizes the Dysfunctional Immune Regulation in Multiple Sclerosis
62 Citations2010Nicholas Schwab, Alla L. Zozulya +3 more
Trends in Molecular MedicineGene expression profiles in peripheral blood for the diagnosis of autoimmune diseases
51 Citations2011Bertalan Meskó, Szilárd Póliska +1 more
It is suggested that peripheral blood mononuclear cells are suitable for descriptive and comparative gene expression analyses and a general protocol for future studies and decision tree for researchers are presented to facilitate standardization and adoption of this approach.
Immunology and Cell BiologyA CD2 high‐expressing stress‐resistant human plasmacytoid dendritic‐cell subset
40 Citations2016Christian Bryant, Phillip D. Fromm +14 more
The selective apoptosis of CD2lo pDC during stress represents a novel mechanism for the control of innate responses in human plasmacytoid dendritic cells.
Protein & CellHuman BDCA2+CD123+CD56+ dendritic cells (DCs) related to blastic plasmacytoid dendritic cell neoplasm represent a unique myeloid DC subset
32 Citations2015Haisheng Yu, Peng Zhang +9 more
It is proposed that the CD4+CD56+ neoplasm may be a tumor counterpart of CD56+ mDCs but not pDCs, a novel mDC subset mixed with some pDC features.
NatureCancer vaccine triggers antiviral-type defences
28 Citations2016I. Jolanda M. de Vries, Carl G. Figdor
Statistical Applications in Genetics and Molecular BiologyThe Joint Null Criterion for Multiple Hypothesis Tests
26 Citations2011Jeffrey T. Leek, John D. Storey
A criterion defining a well behaved set of simultaneously calculated p-values that provides precise control of common error rates is proposed and diagnostic procedures for assessing whether the criterion is satisfied with simulations are introduced.
The Journal of ImmunologyA 17q12 Allele Is Associated with Altered NK Cell Subsets and Function
25 Citations2012Zongqi Xia, Qingquan Liu +8 more
The rs9916629C allele has a validated effect on the proportion of NK cells in peripheral blood and skews NK cells toward a specific phenotypic and functional profile, potentially influencing the impact that these innate immune cells have on infection and autoimmunity.
Scientific ReportsCharacterization of species-specific genes regulated by E2-2 in human plasmacytoid dendritic cells
19 Citations2015Menglan Cheng, Xuyuan Zhang +6 more
It is highlighted that E2-2 modulates pDC function in a species-specific manner, which may provide insights for pDC development and functions.
PLoS ONECharacterization of CD56+ Dendritic-Like Cells: A Normal Counterpart of Blastic Plasmacytoid Dendritic Cell Neoplasm?
17 Citations2013Yohei Osaki, Akihiko Yokohama +14 more
The results indicate that pDLCs might be immature DCs and might contribute to the immunophenotypical diversity of BPDCNs, a rare hematological malignancy.
Clinical ImmunologySiglec-1-positive plasmacytoid dendritic cells (pDCs) in human peripheral blood: A semi-mature and myeloid-like subset imbalanced during protective and autoimmune responses
17 Citations2015Theresa R Wilhelm, Adriano Taddeo +10 more
It is demonstrated by flow-cytometry and confocal microscopy that Siglec-1, a molecule involved in the regulation of adaptive immunoresponses, is expressed in a subset of semi-mature, myeloid-like pDCs in human blood.
BMC BioinformaticsGene signature-based mapping of immunological systems and diseases
9 Citations2016Hong Liu, Jessica Liu +3 more
This modular ‘biomap’ reveals the linkages between different diseases and immune subtypes, and provides evidence for the presence of specific immunocyte subsets in mixed tissues.
Cellular and Molecular ImmunologyPreferential depletion of CD2low plasmacytoid dendritic cells in HIV-infected subjects
5 Citations2011Qiumei Du, Yanmei Jiao +8 more
It was found that the CD2low pDC subset was preferentially depleted in infected individuals, and this finding furthers the understanding of the causes and consequences of pDC depletion during HIV infection.
…